- navigate_next Services
- navigate_next About SPILLO-PBSS
- navigate_next Experimental validations / Publications
Multilevel cross-organism transferability analysis (MCOTA)
Through a multilevel cross-organism transferability analysis (MCOTA) between Homo sapiens and other organisms (e.g., Rattus norvegicus), and vice versa, SPILLO-PBSS makes it possible to assess the reliability of an animal model in advance, for an improved rational design of animal testing and better interpretation of the results.
|Possible checks and evaluations:|
|1||Checking whether the SPILLO-PBSS identified (e.g., in Homo sapiens) potential target proteins of a xenobiotic are also present in the model organism (e.g., Rattus norvegicus)|
|2||Calculating the overall sequence identity between the human target protein and the same protein in the model organism|
|3||Verifying the local similarity between the xenobiotic binding site within the human target protein and the corresponding target protein of the model organism (e.g., by using SPILLO-PBSS to analyze a 3D model of the model organism protein).|
If carried out in advance, MCOTA makes it possible to avoid unnecessary experiments and to design animal testing in a more targeted way, according to the 3Rs guiding principles. Vice versa, once identified the target proteins of a xenobiotic in a model organism (e.g., Rattus norvegicus) by SPILLO-PBSS, the same analysis can be carried out to assess the transferability of the results to Homo sapiens.
A representative example of MCOTA can be found in the experimental tests performed to validate a SPILLO-PBSS identified off-target of finasteride (see Paper).